recombinant human il 6 protein  (MedChemExpress)

Journal: International Journal of Molecular Medicine

Article Title: IL-37/IL-1R8 blocks keratinocyte acantholysis via suppressing ADAM17/EGFR

doi: 10.3892/ijmm.2026.5793

Figure Lengend Snippet: IL-37 protects HaCaT cells from Dsg3-induced keratinocyte dissociation and apoptosis. (A) The effect of different dilutions of Dsg-3 antibodies on cell dissociation was analyzed by immunofluorescence. (B) HaCaT cells were treated with different concentration of IL-37 recombinant protein (0, 25, 50, 100, and 200 ng/ml). Cell viability was detected by CCK-8 assay. (C) HaCaT cells were treated with the Dsg-3 antibodies, followed by administration of the IL-37 recombinant protein. The concentration of IL-37 was analyzed using an ELISA assay. (D) The relative mRNA expression of IL-37 was detected using RT-qPCR. The (E) concentration and (F) relative mRNA expression of IL-10 were measured using an IL-10 ELISA kit and RT-qPCR, respectively. The (G) oncentration and (H) relative mRNA expression of IL-6 were measured by the IL-6 ELISA kit and RT-qPCR, respectively. The (I) concentration and (J) relative mRNA expression of IL-17 were measured by the IL-17 ELISA kit and RT-qPCR, respectively. & P<0.05; * P<0.05 vs. Control group; # P<0.05 vs. anti-Dsg3 group. Data are presented as mean ± SD, n=3 biological independent replicates. One-way ANOVA with Bonferroni's post-hoc test was used for multiple group comparisons. IL, interleukin; Dsg3, desmoglein-3; ELISA, enzyme-linked immunosorbent assay; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Subsequently, the cells were treated with IL-37 recombinant protein (isoform b; 100 ng/ml; cat. no. 10155-HNAE; Sino Biological, Inc.) for 24 h at 37°C.

Techniques: Immunofluorescence, Concentration Assay, Recombinant, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Medicine

Article Title: IL-37/IL-1R8 blocks keratinocyte acantholysis via suppressing ADAM17/EGFR

doi: 10.3892/ijmm.2026.5793

Figure Lengend Snippet: IL-37/IL-1R8 protects HaCaT cells from keratinocyte dissociation and apoptosis through the ADAM17/EGFR pathway. (A) The interaction between IL-37 and IL-1R8 was analyzed using co-immunoprecipitation. (B) HaCaT cells were transfected with IL-1R8 siRNA and the transfection efficiency were detected by western blotting. (C) HaCaT cells were transfected with IL-18Rα siRNA and the transfection efficiency were detected by western blotting. (D) IL-1R8 siRNA or IL-18Rα transfected HaCaT were treated with anti-Dsg-3 and IL-37 recombinant protein. The number of fragments was analyzed by cell dissociation assay. (E and F) Cell apoptosis was analyzed by flow cytometry. (G) Protein expression levels of Bcl-2 and Bax were determined by western blotting. & P<0.05 vs. si-NC; * P<0.05 vs. anti-Dsg3 + IL-37 + si-NC group. Data are presented as mean ± SD, n=3 biological independent replicates. One-way ANOVA with Bonferroni's post-hoc test was used for multiple group comparisons. IL, interleukin; ADAM17, TNF-alpha-converting enzyme; EGFR, epidermal growth factor receptor; si, short interfering.

Article Snippet: Subsequently, the cells were treated with IL-37 recombinant protein (isoform b; 100 ng/ml; cat. no. 10155-HNAE; Sino Biological, Inc.) for 24 h at 37°C.

Techniques: Immunoprecipitation, Transfection, Western Blot, Recombinant, Flow Cytometry, Expressing

Journal: Oncology Reports

Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation

doi: 10.3892/or.2026.9086

Figure Lengend Snippet: IL-6 abolishes the effects of CCT2 knockdown on the proliferation and invasion of hepatocellular carcinoma cells. The protein levels of STAT3 and p-STAT3 in (A) Huh-7 and (B) HCCLM3 cells were measured by western blotting. The proliferation of (C) Huh-7 and (D) HCCLM3 cells following CCT2 knockdown and IL-6 treatment was detected using EdU incorporation assay. The invasion of (E) Huh-7 and (F) HCCLM3 cells was assessed using Transwell assay. *P<0.05, **P<0.01 vs. PBS. CCT2, chaperonin containing TCP1 subunit 2; sh, short hairpin; p-, phosphorylated.

Article Snippet: Recombinant human IL-6 (cat. no. HY-P7044; MedChemExpress) as used for STAT3 activation.

Techniques: Knockdown, Western Blot, Transwell Assay

Journal: Acta Otorhinolaryngologica Italica

Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

doi: 10.14639/0392-100X-A1222

Figure Lengend Snippet: Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Acta Otorhinolaryngologica Italica

Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

doi: 10.14639/0392-100X-A1222

Figure Lengend Snippet: The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

Techniques: Blocking Assay, Transwell Assay, Migration, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing